Mapping of the amino-terminal interaction domain within ELL.

A series of deletion mutants containing different regions of the amino-terminus of ELL were cloned in the pFLAG vector and transiently transfected in 293 cells. The top panels demonstrate expression of these constructs with the FLAG antibody. The cell lysates were immunoprecipitated with the FLAG antibody and probed with the EAF1 and EAF2 monoclonal antibodies. The bands corresponding to the endogenous EAF1 and EAF2 proteins that coprecipitated with the transfected FLAG-tagged ELL constructs are indicated with an arrowhead.