EAF2 contains a transactivation domain in its carboxy-terminus.

(A) Western blot of protein extracts from 293 cells transfected with GAL4-EAF2 and GAL4-EAF1 fusion proteins and detected with the GAL4 antibody. (B) Transcriptional activation assay. The indicated fold activation levels are relative to those of the controls. Each bar represents the mean ± standard deviation of at least 3 independent experiments. GAL4-EAF2 constructs are shown in light boxes (■) and the corresponding GAL4-EAF1 constructs in gray boxes (░). The columns represent the following constructs: GAL4-EAF2 1-260 and GAL4-EAF1 1-268, GAL4-EAF2 1-96 and GAL4-EAF1 1-90, GAL4-EAF2 86-176 and GAL4 EAF1 91-181 GAL4-EAF2 177-260, and GAL4-EAF1 182-268. Results with the GAL4-E1bCAT promoter are on the left and the GAL4-tkCAT promoter on the right.