GILZ gene expression in normal human tissues.

GILZ gene expression was studied by in situ hybridization in the lungs (A-B), liver (C), and kidneys (D), with either an antisense (A, C-D) or a sense (B) GILZ probe. In lung samples, macrophages were identified by immunohistochemistry with anti-CD68 antibodies (E), and GILZ gene–expressing cells were identified by double-labeling experiments in which in situ hybridization with use of the GILZ antisense probe (dark blue) was combined with immunohistochemistry with an anti-CD68 antibody (brown; F). The results shown are representative of experiments performed on 3 different lungs, livers, and kidneys. Original magnifications A-B, × 400; C, × 250; inset C, × 400; D, × 400; E-F, × 1600; inset F, × 2000.