In vivo induction of GILZ in mice.

(A) Production of GILZ was evaluated in mice treated with PBS (control, n = 6) or with DXM (n = 6). GILZ gene expression was measured by RT-PCR. (i) Optical density of products amplified from GILZ mRNA (means ± SEMs), *P < .01 (Mann-WhitneyU test). ■ indicates control; ▪, treated with DXM. (ii) A typical result for analysis of GILZ gene expression in the lung from 3 control and 3 DXM-treated mice. 0: negative control, with no cDNA. (iii) Presence of GILZ and β-tubulin in the lung of control and DXM-treated mice, as analyzed by Western blotting. Results shown are from 1 experiment representative of 2. (B) In vitro and (C) in vivo GILZ gene expression by peritoneal macrophages were evaluated by RNase protection assay (RPA). (Bi, Ci) Lanes 1 and 2: undigested β-actin and GILZ probes, respectively; lane 3: RPA in the presence of yeast RNA. (Bi) Lanes 4 and 5: macrophages cultured alone and with DXM, respectively. (Ci) Lanes 4 and 5: macrophages from control and DXM-treated mice, respectively. The ratios between protected GILZ and β-actin probes for lanes 4 and 5 are shown in panels Bii and Cii.