American Society of Hematology

Fig. 9.

$Fig. 9. GILZ inhibits TLR2 expression. / (A) THP-1 cells were cultured with or without DXM or IL-10. (B) THP-1 cells were transfected with the control (pcDNA3) or the GILZ-encoding vector. Cells were stimulated with LPS, IFNγ, or an anti-CD40 mAb. TLR2 expression was determined at the end of culture. Open histograms correspond to the staining obtained for the control Ab, and solid histograms correspond to cells labeled with the anti-TLR2 Ab. Results are from 1 experiment of 4. In the upper right-hand corner of each histogram, the mean fraction (%) of cells expressing TLR2 in these 4 experiments is shown. In cells cultured without LPS, IFNγ, or anti-CD40 mAb, 6.3% ± 1.0% of the cells expressed TLR2.$

GILZ inhibits TLR2 expression.

(A) THP-1 cells were cultured with or without DXM or IL-10. (B) THP-1 cells were transfected with the control (pcDNA3) or the GILZ-encoding vector. Cells were stimulated with LPS, IFNγ, or an anti-CD40 mAb. TLR2 expression was determined at the end of culture. Open histograms correspond to the staining obtained for the control Ab, and solid histograms correspond to cells labeled with the anti-TLR2 Ab. Results are from 1 experiment of 4. In the upper right-hand corner of each histogram, the mean fraction (%) of cells expressing TLR2 in these 4 experiments is shown. In cells cultured without LPS, IFNγ, or anti-CD40 mAb, 6.3% ± 1.0% of the cells expressed TLR2.

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