Fig. 5.
![Fig. 5. SDF-1–induced calcium mobilization. / Stably transfected HEK/CD4.403 cells expressing wild-type and mutated CXCR4 receptors were loaded with Fluo-3-AM. SDF-1–induced calcium flux was measured as described in “Materials and methods” in the presence (○) or absence (●) of PTX preincubated overnight at 100 ng/mL. Representative curves of [Ca2+]i changes after stimulation with SDF-1α and ionomycin (I) from 1 to 4 experiments are shown.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/101/2/10.1182_blood-2002-03-0978/4/h80233648005.jpeg?Expires=1736050863&Signature=1hrI1Z8f2-N94AqSRQ5uLc3xbt0XagZtdQTymvKHAilIMTYRLm3w5hm3lfl7puruR6erkuG6bvcoxS1yFbk1VZCNrFB3Dlc-ZvM6dLbhCss9sfXSShqtuaTI09fCzR5F33IOGtcd1q8oEtPp8cLPswLxBmVv0RlipPsjdXPw7McRHzS~bZ39YqFIys0OdZEDUdDXik9pMwQROurTFeKm-IkMKTDiE926RJiGgeWT-XQW4kNvT5avH4~JxvV8hd8ziC-vila4BPCacztmIzSydzdlgyxbzi6hN3mDO21Tfxo86CXIrOds4G~z5sQ~JAhy7Ucn270GPp7I0GiaI~XS8A__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
SDF-1–induced calcium mobilization.
Stably transfected HEK/CD4.403 cells expressing wild-type and mutated CXCR4 receptors were loaded with Fluo-3-AM. SDF-1–induced calcium flux was measured as described in “Materials and methods” in the presence (○) or absence (●) of PTX preincubated overnight at 100 ng/mL. Representative curves of [Ca2+]i changes after stimulation with SDF-1α and ionomycin (I) from 1 to 4 experiments are shown.
