Visible alterations in the mouse mesenteric microcirculation following I/R of the SMA.

Images are representative pictures of the mouse mesenteric microcirculation as observed in sham-operated mice (A,C) and following occlusion and reopening of the SMA (30 minutes + 45 minutes) (B,D). (A) Clear example of a noninflamed vessel in the sham group as observed 75 minutes after laparoctomy. Arrow and arrowhead highlight 2 mast cells in the extravascular tissue, whereas segment illustrates the canonical 100 μm vessel length. (B) Typical signs of leukocyte recruitment as seen 45 minutes after reperfusion, with cells adherent to the postcapillary venule endothelium (arrow) and migrated into the extravascular space (arrowheads). Segment illustrates depth (50 μm) into the extravascular tissue. (C) In sham-operated mice, most fluorescent albumin is found intravascularly. (D) Example of diffuse fluorescence in mesenteries subject to I/R. Pictures are representative of n = 4 to 8 mice per group. Original magnification, × 400.