Peptide Ac2-26 and ATLa cause detachment of adherent leukocytes.

Mouse mesenteries were subject to 30 and 45 minutes of I/R procedure. At time 0, with the postcapillary venule exposed, peptide Ac2-26, ATLa, or KC was given intravenously in a 100 μL volume. The fate of the adherent leukocytes was monitored for an additional 10 minutes. (A) Effect of peptide Ac2-26 (100 μg) injected into WT (■) or FPR KO (▪) mice on the number of cells adherent at time 0 and remained adherent 10 minutes after treatment. (B) Effect of ATLa (10 μg, ■), fMLP (10 μg, ▨), and KC (1 μg, ▪) injected into FPR KO mice on the fate of cells adherent at time 0, as measured 10 minutes later. (C) Effect of Ac2-26 (100 μg), ATLa (10 μg), fMLP (10 μg), or KC (1 μg) on the number of newly recruited adherent cells within the postcapillary venule endothelium under observation. (D) Wall shear rate (WSR) values of the vessels analyzed in panels A-C. Data are mean ± SEM of n = 6 mice (peptide Ac2-26 in WT or FPR KO; ATLa and fMLP in FPR KO) or n = 3 mice (KC in FPR KO) per group. *P < versus baseline (time 0) values.