Transduced HSCs give rise to a high frequency of mature T and B cells in bone marrow chimeras.

FACS analyses were performed on splenocytes from RAG-2 KO recipients that received transplants of either WT or WKO HSCs transduced with the indicated retroviruses (presorted for GFP expression prior to transplantation). GFP expression confirms that the lymphocytes arose from the transplanted HSCs. Upper and lower panels reveal the percentage of transduced T cells (Thy1⁺ GFP⁺) and B cells (B220⁺GFP⁺), respectively. Splenocytes from a naive WT mouse served as a control.