Retrovirus-mediated WASP-gene transfer rescues T-cell signaling defects in WKO mice.

Lymphocytes isolated from RAG-2 KO recipient mice that received transplants of the indicated HSCs (keys) were stimulated with either anti-CD3 antibodies (A, 0-12 mg/mL; C, 12 mg/mL) or control PMA/ionomycin (B,D). The error bars represent the standard error. Two representative experiments are shown (A-B and C-D). In the first experiment (A-B), HSCs were not presorted for GFP expression prior to transplantation; in the second experiment (C-D), HSCs were presorted for GFP expression prior to transplantation. Proliferation as measured by [³H]thymidine incorporation is shown on the ordinate. Equal levels of proliferation in response to PMA/ionomycin (B,D) are used as a control for cell numbers with cells stimulated with anti-CD3 antibodies (A,C). Note in panels A and C that WKO cells transduced with CMMP-vectors expressing WASP-GFP but not GFP alone partially rescue the T-cell–signaling defect. N-WASP expression can also partially rescue defective T-cell signaling in WKO cells (C).