Activation, transduction, and selection procedures result mainly in IL-2 and IFN-γ–producing T cells, documenting a preferential skewing toward CD4⁺ T_H1 and CD8⁺ T_C1 phenotypes.

Intracytoplasmic staining of RV cells for IFN-γ (FITC; x axes, all panels), IL-2 (PE; y axes, left panels), IL-4 (PE; y axes, central panels), and IL-10 (PE; y axes, right panels) is shown. Results were analyzed gating CD4⁺ (upper panels) and CD8⁺ (lower panels). Quadrant markers were positioned to include 95% of stained, unstimulated cells in the lower left square (upper panel of each series). Results from 1 of 3 representative experiments are shown.