Fig. 3.
Fig. 3. CTLs specific for CMV pp65 are preserved in RV cells. / T cells specific for the immunodominant HLA-A0201–restricted pp65495-503 peptide derived from the matrix protein pp65 of CMV were detected by staining with a QR-conjugated CD8 and a PE-conjugated pp65 tetramer followed by cytofluorometric analysis. Upper panels show the expression of ΔLNGFR in PBLs (upper left panel) and RV cells (upper right panel). Middle panels show FL-2 autofluorescence of cells stained with QR-conjugated CD8 antibody and FITC-ΔLNGFR antibody. Lower panels show the percentage of QR-CD8+/PE-pp65-tetramer+ T cells in PBLs.

CTLs specific for CMV pp65 are preserved in RV cells.

T cells specific for the immunodominant HLA-A0201–restricted pp65495-503 peptide derived from the matrix protein pp65 of CMV were detected by staining with a QR-conjugated CD8 and a PE-conjugated pp65 tetramer followed by cytofluorometric analysis. Upper panels show the expression of ΔLNGFR in PBLs (upper left panel) and RV cells (upper right panel). Middle panels show FL-2 autofluorescence of cells stained with QR-conjugated CD8 antibody and FITC-ΔLNGFR antibody. Lower panels show the percentage of QR-CD8+/PE-pp65-tetramer+ T cells in PBLs.

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