HFE does not alter TfR-TfR2 interaction.

Cell extracts from K562 cells were incubated with 1 μM purified recombinant HFE for 60 minutes at 4°C (K562 + HFE) and immunoprecipitated using sheep anti-TfR serum andS aureus (IP:TfR). Immunoprecipitated proteins were subjected to SDS-PAGE on an 8% gel, transferred to nitrocellulose, and immunodetected for TfR2 (upper panel) (WB:TfR2) and TfR (lower panel) (WB:TfR). NS indicates background control using K562 cell extracts and S aureus without antibody; vertical dash, no cell extract with anti-TfR and S aureusonly; and CE, cell extract.