AZT inhibits constitutive NF-κB nuclear activity in PEL.
(A) NF-κB complexes in primary PEL (BCLM) and BCBL-1 are composed of p50/p65 heterodimer. Purified nuclear extracts (15 μg) from PEL cells were preincubated with the indicated antibodies and subjected to EMSA using the 32P-labeled NF-κB oligonucleotide probe. The results are representative of 3 independent experiments. (B) AZT blocks NF-κB nuclear colocalization in PEL. Nuclear extracts were prepared from cells treated with AZT (10 μg/mL) for the indicated times, and binding activity was assayed by EMSA using either NF-κB (top panels) or AP-1 (bottom panels) specific consensus oligonucleotides as probes. (C) Induction of NF-κB by IFN-α and soluble TRAIL in BCBL-1. BCBL-1 cells were treated with either IFN-α (1000 U/mL) for 18 hours or soluble TRAIL (50 ng/mL) for 1 hour. Nuclear extracts were then assayed by EMSA for NF-κB. (D) Effect of nucleoside analogs and BAY-11 on NF-κB translocation. BCBL-1 and BCLM cells were treated with AZT (10 μg/mL), BAY-11 (10 μM), ddI (10 μg/mL), or ddC (10 μg/mL) for 2 hours and assayed by EMSA using NF-κB consensus oligonucleotides as probes.
