TfR and TfR2 under nonreducing conditions.

Cell extracts (25 μg) from TfR-transfected TRVb cells (TRVb1) and K562 cells were separated on an 8% acrylamide gel under denaturing but nonreducing conditions, transferred to nitrocellulose, and Western blotted for TfR and TfR2. TfR homodimer is approximately 190 kDa, while the TfR2 homodimer is a doublet of approximately 200 and 210 kDa.