American Society of Hematology

Fig. 4.

$Fig. 4. AZT is phosphorylated in PEL cells. / (A) Primary PEL cells (BCLM) phosphorylate AZT to AZTMP. A total of 5 × 106 BCLM cells were treated with 3H-AZT and cold AZT (5 μg/mL) for 3 and 24 hours. Methanol extracts were then subjected to HPLC fractionation to separate the different forms of AZT. White bar (■) indicates AZT; gray bar (░), AZTMP; black bar (▪), AZTDP; and vertical lined bar (▥), AZTTP. (B) Quantification of total phosphorylation of AZT in HHV-8+ PEL cells versus viral negative B-cell lymphoma (BJAB). A total of 5 × 106 HHV-8+ PEL (BCLM, BCBL-1) and BJAB cells were treated with 3H-AZT and cold AZT (5 μg/mL) for 3 and 24 hours. Methanol extracts were then subjected to HPLC, and all forms of phosphorylated AZT were quantitated by β-scintillation counting. White bar (■) indicates BJAB; gray bar (░), BCLM; and black bar (▪), BCBL-1.$

AZT is phosphorylated in PEL cells.

(A) Primary PEL cells (BCLM) phosphorylate AZT to AZTMP. A total of 5 × 10⁶ BCLM cells were treated with ³H-AZT and cold AZT (5 μg/mL) for 3 and 24 hours. Methanol extracts were then subjected to HPLC fractionation to separate the different forms of AZT. White bar (■) indicates AZT; gray bar (░), AZTMP; black bar (▪), AZTDP; and vertical lined bar (▥), AZTTP. (B) Quantification of total phosphorylation of AZT in HHV-8⁺ PEL cells versus viral negative B-cell lymphoma (BJAB). A total of 5 × 10⁶ HHV-8⁺ PEL (BCLM, BCBL-1) and BJAB cells were treated with ³H-AZT and cold AZT (5 μg/mL) for 3 and 24 hours. Methanol extracts were then subjected to HPLC, and all forms of phosphorylated AZT were quantitated by β-scintillation counting. White bar (■) indicates BJAB; gray bar (░), BCLM; and black bar (▪), BCBL-1.

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