Expression of wild-type or mutated murine PSGL-1 on transfected CHO cells coexpressing Core2GlcNAcT-I and FTVII.

The cells were permanently transfected with an empty expression vector (control) or with an expression vector encoding wild-type murine PSGL-1 or the indicated mutant PSGL-1 construct. The cells were incubated with antimurine PSGL-1 mAb 2PH1. Bound mAb was detected with FITC-conjugated rat antimouse IgG1 mAb. Incubation of PSGL-1–transfected cells with FITC-conjugated rat antimouse IgG1 mAb alone gave the same low background staining as observed with the cells transfected with the empty expression vector. The data are representative of at least 5 experiments.