Fig. 5.
Fig. 5. Effect of inhibiting sulfation of murine PSGL-1 on binding to P-selectin. / CHO cells expressing Core2GlcNAcT-I, FTVII, and either wild-type or Tyr13/15Phe murine PSGL-1 were cultured in the presence or absence of 100 mM sodium chlorate for 72 hours. (A) Binding of P-selectin/IgM was measured by flow cytometry as in Figure 3. The data are representative of 3 experiments. (B) The number of cells rolling on P-selectin was measured as in Figure 4A. The data represent the mean ± SEM of 3 experiments.

Effect of inhibiting sulfation of murine PSGL-1 on binding to P-selectin.

CHO cells expressing Core2GlcNAcT-I, FTVII, and either wild-type or Tyr13/15Phe murine PSGL-1 were cultured in the presence or absence of 100 mM sodium chlorate for 72 hours. (A) Binding of P-selectin/IgM was measured by flow cytometry as in Figure 3. The data are representative of 3 experiments. (B) The number of cells rolling on P-selectin was measured as in Figure 4A. The data represent the mean ± SEM of 3 experiments.

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