Structure and expression pattern of the mouse

TM9SF2 and mTAB2 genes.(A) Schematic representation of the mouse TM9SF2 gene locus. The 2746-bp mRNA sequence of mTM9SF2 (BC003862) was aligned against the Celera mouse genome database to retrieve information about the genomic structure. The gene contains at least 17 exons spanning approximately 53 kb and is located at mouse chromosome 14. The human and mouse TM9SF2 genes have a similar structure, and the hTM9SF2 is located on chromosome 13q11-34. (B) Northern blot with RNA derived from several adult mouse tissues probed with mTM9SF2 probe. Specific expression in all tissues is observed, with the highest expression in liver and kidney. The probe hybridizes to an RNA of approximately 3 to 3.5 kb. (C) Complementary DNA dot blot analysis shows mTM9SF2 andmTAB2 expression in several hematopoietic cell lines. FormTM9SF2, the highest expression levels were observed in myeloid (32D.cl3, DA3) and B-lymphoid (DA8) and lowest expression in T-lymphoid (DA2) and erythroid (MEL) cell lines. For mTAB2the highest levels of expression were observed in the myeloid cell lines. (D) Schematic representation of the mTAB2 gene locus. The 3480 bp mRNA sequence (BC004813) was aligned against the Celera mouse genome database to retrieve information about the genomic structure. The gene contains at least 6 exons spanning approximately 20 kb and is located at mouse chromosome 10. The gene structure ofmTAB2 and hTAB2 is similar, and hTAB2is located on human chromosome 6q25. (E) Northern blot containing RNA from E10 and E11 AGM and E11 liver tissues probed with anmTAB2 probe. The probe hybridizes to an RNA of approximately 3.5 to 4 kb. Differential expression of mTAB2 in the AGM between E10 and E11 is observed. (F) Northern blot containing RNA from adult mouse tissues reveals high levels of mTAB2 expression in the kidney, thymus, spleen, and BM.