Transduction of freshly isolated myeloma B cells.

Myeloma cells from 3 of the 6 patients (see “Results”) are shown. Cells were isolated and cultured with IL-6 and GM-CSF. HIV-CMV vector packaged with integrase wild-type (integrase +) or mutant (integrase −; one myeloma) plasmid was added 24 hours after culture initiation. Vector amounts were normalized using reverse transcriptase activities and corresponded to an MOI of 10. Percentages of GFP⁺ cells among gated CD138⁺7-AAD^low cells were measured after 5 days (the myeloma shown at the top, single culture) or 4 days (the 2 other myeloma; duplicate cultures with integrase + vector gave 28%; 25% and 65%; 61% GFP⁺ cells).