Incorporation of 4-F-Glc[³H]NAc into TCA-precipitated macromolecules isolated from human CLA⁺ T cells.

Cellular macromolecules were precipitated from 4-F-Glc[³H]NAc–treated CLA⁺ T cells (0-1.0 mM or 0-16.7 μCi/mL [0-0.6179 MBq/mL] for 36 hours or less) with 10% TCA and collected on GF/C microfiber glass filters for liquid scintillation counting. Background counts per minute from non–4-F-Glc[³H]NAc–treated cells were subtracted from counts per minute measured from TCA precipitates of 4-F-Glc[³H]NAc–treated cells, and data in triplicate were expressed as nmol 4-F-Glc[³H]NAc incorporation per cell (SEM) at 12, 24, and 36 hours. Please note that 4-F-Glc[³H]NAc was detectable in TCA precipitates in a concentration-dependent manner over a 24-hour period.