C/EBPα inhibits erythrocyte differentiation and leads to granulocytic differentiation.

(A) Hematopoietic cells transduced with MIGR1 or MIGR1 C/EBPα-ER were sorted for expression of GFP and plated in clonogenic assays in triplicate with or without β-estradiol. Colonies were scored and counted 12 to 14 days later. One of 2 representative experiments is shown. (B) Transduced hematopoietic cells were sorted for GFP and CD71 expression higher than 10² mean fluorescence intensity (CD71^bright) and plated in clonogenic assays in duplicate. Shown is 1 of 3 independent experiments. (C) Cells were collected from the clonogenic assays (see Figure 2B) and analyzed for surface marker expression (CD11b, CD14, and glycophorin A) by flow cytometry. Cytospins were performed and stained with Wright-Giemsa (last panels on the right; original magnification, × 600). Cells transduced with MIGR1 and treated with ethanol or β-estradiol showed the same phenotype as cells transduced with C/EBPα-ER and treated with ethanol (data not shown).