LPS induction of Egr-1, PAI-1, ICAM-1, and TF mRNAs in the kidney.

(A) Egr-1^+/+ mice (2 per group) were either untreated (0 hours) or injected intraperitoneally with LPS (2 mg/kg) for various times (0.5 to 8 hours). Egr-1, PAI-1, ICAM-1, and TF mRNA levels were measured by Northern blotting. G3PDH was used as a loading control. (B) LPS induction (2 hours) of Egr-1 protein was detected by immunoprecipitation and Western blotting.