LPS induction of Egr-1 and TF chemokine expression in the lungs of Egr-1^+/+ and Egr-1^−/− mice.

(A) (Left panel) Egr-1^+/+ mice (2 per group) were either untreated (0 hours) or injected intraperitoneally with LPS (2 mg/kg) for various times (0.5 to 4 hours). (Right panel) LPS induction of Egr-1 mRNA and protein (2 hours) was detected by Northern and Western blotting, respectively. (B) Egr-1^+/+ mice (3 per group) were either untreated or injected with LPS for 8 hours. In a second experiment, Egr-1^+/+ and Egr-1^−/− mice (3 per group) were injected with LPS for 8 hours. TF mRNA levels were determined by Northern blotting. (C) Various chemokine mRNAs were measured in the lungs of untreated and LPS-treated Egr-1^+/+and Egr-1^−/− mice by RPA using the mouse chemokine multiprobe template set mCK-5 (Pharmingen). This probe set (left lane of each gel) contains Ltn, RANTES, MIP-1β, MIP-1α, IL-10, MIP-2, MCP-1, and TCA-3. G3PDH was used as a loading control. Normalized levels of TF mRNA (mean ± SD) and MCP-1 mRNA (mean ± SE) are shown below. The asterisk indicates that the decrease was statistically significant (P < .05).