LV'VFas⁺ T cells are sensitive to AP1903-induced cell death in vitro.

(A) Representative dose-response curve of AP1903-induced killing of primary LV'VFas⁺ T cells after purification for ΔLNGFR expression. Aliquots of macaque LV'VFas⁺ T cells were exposed for 2 hours with the indicated concentrations of AP1903 (●) or medium alone (○) and evaluated 24 hours later by trypan blue exclusion. The result shown is representative of 10 independent experiments with human or macaque T cells, indicating maximal killing at 5 nM. (B) Repeated exposure of LV'VFas⁺ T cells to AP1903 will eliminate the majority of LV'VFas-modified T cells. Aliquots of LV'VFas⁺ T cells were exposed once or 4 times (48 hours apart) for 2 hours to medium alone (■) or to medium containing 10 nM AP1903 (▪). Cells were washed, and survival was evaluated 24 hours later by trypan blue exclusion.