The TCR repertoire is maintained in cultures of transduced and immunomagnetic-selected LV'VFas-modified T cells.

(A) Spectratype profile for TCR Vβ11 and TCR Vβ23 from a single individual in response to stimulation to different activation methods. Multiplex PCR analysis of TCR Vβ gene usage in freshly isolated human PBLs and LV'VFas⁺ T cells cultured for 14 days was performed as described in “Materials and methods.” The profiles are of PBLs before and of LV'VFas⁺ T cells after stimulation with anti-CD3 and anti-CD28, or anti-CD3 alone. The horizontal axis denotes the length in base pairs. The data are representative of 2 separate experiments performed for comparison. (B) Representative flow cytometric analysis of the TCR Vβ gene usage in PBLs (▧) and unmodified T cells (■), or LV'VFas-transduced T cells either unselected (■) or ΔLNGFR-enriched (▪). Cells were stained with a panel of 24 human anti–TCR Vβ and anti-CD3 mAbs and evaluated by flow cytometry. The percentage of CD3⁺ cells positive for the individual TCR Vβ mAbs are as indicated.