LV'VFas⁺ T cells retain responses to antigen derived from pathogens and to allogeneic cells.

(A) Freshly isolated PBLs (▪) and anti-CD3– and anti-CD28–stimulated T cells either unmodified (■), LV'VFas-transduced but unselected (▧), or LV'VFas⁺(░) were stimulated with PHA, or antigen derived from CMV, varizella-zoster virus (VZV), or candida, respectively, or with media alone. Cultures were pulsed with [³H]thymidine 16 hours prior to harvest. The [³H]thymidine incorporation was determined in counts per minute (cpm). The stimulation index was calculated by the ratio of cpm incorporated in wells stimulated with antigen to cpm in wells stimulated with medium alone. (B) Freshly isolated PBLs (▪) and T cells either unmodified (■) or LV'VFas⁺ (░) were plated with γ-irradiated HLA-disparate PBLs from 2 donors either in medium alone or in medium containing 100 ng/mL cyclosporin A (CSP) in a conventional mixed lymphocyte culture as described in “Materials and methods.” The stimulation index was calculated as described above. The data shown are from 1 of 3 separate experiments with different donors.