SU5614 induces growth arrest in Ba/F3 cells transformed by FLT3ITD and FLT3D835Y mutants in the absence but not in the presence of IL-3.

(A) SU5614 induces growth arrest in Ba/F3 cells transformed by FLT3ITD-NPOS, FLT3ITD-W51, FLT3D835Y, but not by TEL-ABL. Mean values and SDs were calculated from 3 independent experiments. The growth of cells in the absence of inhibitor at 72 hours was defined as 100%. (B) Dose-response curve of the inhibitory activity of SU5614 on Ba/F3 cells transformed by FLT3ITD-NPOS and FLT3D835Y after 72 hours of incubation. The growth of cells that were incubated without inhibitor was defined as 100%. Values represent means and SDs from 3 independent experiments.