Fig. 4.
Fig. 4. SU5614 inhibits the tyrosine phosphorylation of FLT3, STAT5, and MAPK in a time- and dose-dependent manner. / (A) Ba/F3 cells expressing FLT3ITD-NPOS were incubated for 6 hours with the indicated concentrations of SU5614 and protein lysates were immunoprecipitated (IP) with FLT3 antibody. Precipitates were analyzed by Western blot. (B) Ba/F3 cells expressing FLT3ITD-NPOS were incubated for the indicated time intervals with SU5614 at a concentration of 1 μM. Protein lysates were subjected to sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) and after blotting, membranes were incubated with the indicated antibodies. (C) Lysates were prepared as described in panel A and membranes were probed with phospho-specific antibodies against STAT5 and MAPK followed by specific STAT5 and MAPK antibody.

SU5614 inhibits the tyrosine phosphorylation of FLT3, STAT5, and MAPK in a time- and dose-dependent manner.

(A) Ba/F3 cells expressing FLT3ITD-NPOS were incubated for 6 hours with the indicated concentrations of SU5614 and protein lysates were immunoprecipitated (IP) with FLT3 antibody. Precipitates were analyzed by Western blot. (B) Ba/F3 cells expressing FLT3ITD-NPOS were incubated for the indicated time intervals with SU5614 at a concentration of 1 μM. Protein lysates were subjected to sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) and after blotting, membranes were incubated with the indicated antibodies. (C) Lysates were prepared as described in panel A and membranes were probed with phospho-specific antibodies against STAT5 and MAPK followed by specific STAT5 and MAPK antibody.

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