The FLT3 PTK inhibitor SU5614 induces growth arrest in AML cell lines expressing an activated FLT3, but not in cells that express a nonactive FLT3 or no FLT3 protein.
(A) Cells were seeded at a density of 1.0 × 105 cells/mL in the absence or presence of different concentrations of SU5614. Viable cells were counted for up to 3 days by trypan blue exclusion. Mean values and SDs were calculated from 3 independent experiments for THP-1 and OCI-AML 5 cells. The growth curve of MM6 cells shows a representative result of 3 experiments with similar results. (B) Dose-response curve of the inhibitory activity of SU5614 in AML cell lines after 72 hours of incubation. The growth of cells that were incubated without inhibitor was defined as 100%. Values represent means and SDs from 3 independent experiments. (C) Dose-response curve of the inhibitory activity of SU5614 in MV4-11 cells after 84 hours of incubation. The OD was measured at 570 nm using an MTT assay (see “Materials and methods”). (D) Growth inhibitory activity of SU5614, SU1498, and imatinib mesylate in MV4-11 and MM6 cells. Cells were seeded at a density of 1 × 105/mL and incubated for 72 hours with 5 μM inhibitor. The growth of cells that were incubated without inhibitor was defined as 100%. Values represent means and SDs from 3 independent experiments.
