Fig. 2.
Fig. 2. Immunolocalization of P2Y1 in platelets activated with ADP. / Unstirred suspensions of washed platelets were incubated at 37°C for 10 minutes with 10 μM ADP in the presence of 400 μg/mL Fg. (A-B) Surface labeling for P2Y1, now includes pseudopods (PSs). Intracellular labeling remains; lines of gold particles (arrows) can be seen. Also recognized by the MoAb is a clear zone in continuity with a thin channel (arrowheads). (C) P2Y1 within a thin channel (arrows) circulating around the centralized granules. Bars = 0.2 μm.

Immunolocalization of P2Y1 in platelets activated with ADP.

Unstirred suspensions of washed platelets were incubated at 37°C for 10 minutes with 10 μM ADP in the presence of 400 μg/mL Fg. (A-B) Surface labeling for P2Y1, now includes pseudopods (PSs). Intracellular labeling remains; lines of gold particles (arrows) can be seen. Also recognized by the MoAb is a clear zone in continuity with a thin channel (arrowheads). (C) P2Y1 within a thin channel (arrows) circulating around the centralized granules. Bars = 0.2 μm.

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