Fig. 3.
Detection of P2Y1 in ADP-desensitized platelets.
Desensitization reverses the changes seen during ADP-induced platelet activation. Platelets were incubated at 37°C with the stable ADP analog ADPβS (1 mM) for 1 hour without stirring in the presence of Fg. Immunolocalization on ultrathin sections of the now discoid platelets showed that the P2Y1 distribution resembled that seen on unstimulated platelets with labeling (arrows) of both surface and internal membrane systems. Platelets are illustrated for 2 healthy donors (A-B). Bar = 0.2 μm.
