Flow cytometric analysis of the binding of an anti-P2Y₁ MoAb to normal platelets.

(A) Experiments were performed with the use of unstimulated PFA-fixed platelets and platelets incubated for 10 minutes with 10 μM ADP before fixation. There was no permeabilization step. (B) The histograms were obtained after permeabilization of PFA-fixed platelets with Triton X-100 and show the intensity of the internal pool of P2Y₁in the internal compartment of unstimulated platelets. The control histograms (neg) were obtained in the presence of irrelevant antibody instead of P2Y₁.