Fig. 7.
Detection of ligand-bound αIIbβ3 by the anti-LIBS MoAb, AP-6.
(A) Unstimulated, washed platelets. Only a few gold particles were present per platelet, and these were located in the vicinity of α-granule membranes (arrow). (B) A similar illustration, but for unstirred platelets incubated with 10 μM ADP for 10 minutes. Note the increased labeling in the vicinity of the α-granule membrane. (C) Double-staining has been performed for platelets incubated with ADP as in panel B; an initiation of secretion is shown by the concomitant labeling with P-selectin (5-nm gold particles) and AP-6 (10-nm gold particles) in a thin channel extending from an α-granule. Bars = 0.1 μm.
