Effect of VX-745 on IL-6 and VEGF secretion in BMSCs.

(A) BMSCs from patients with MM were cultured in the presence (0.06 μM-20 μM) or absence of VX-745 for 48 hours, and cell viability was assessed by MTT assay. The figure represents means (± SDs) from BMSCs of 6 patients with MM. (B) BMSCs were cultured in the presence (0.06 μM-20 μM) or absence of VX-745 for 48 hours. IL-6 (▪) and VEGF (■) in culture supernatants was measured by ELISA. (C) BMSCs were cultured in the presence or absence of SB203580 (2.5 μM-20 μM) for 48 hours. IL-6 (▪) and VEGF (■) in culture supernatants was measured by ELISA. (D) BMSCs were cultured in the presence (0.06 μM-20 μM) or absence of VX-745 for 48 hours. VEGF in culture supernatants was measured by ELISA. (D) BMSCs were cultured with TNF-α (5 ng/mL) in the absence (■) or presence of 0.05 μM (▧), 0.5 μM (▤), and 5 μM (▪) VX-745 for 48 hours. IL-6 in culture supernatants was measured by ELISA.