Fig. 2.
![Fig. 2. Effect of VX-745 on paracrine MM cell growth in the BM. / (A) MM.1S (●), RPMI8226 (▪), and U266 (▴) (3 × 104) cells were cultured in the presence of VX-745 (0.06 μM-20 μM) for 48 hours, and cell viability was assessed by MTT assay. (B) MM.1S cells were cultured in BMSC-coated 96-well plates for 48 hours, in the absence (■) or presence of 0.2 μM (▤), 1 μM (▧), and 5 μM (▪) VX-745. DNA synthesis was assessed by [3H]TdR uptake. (C) BMSCs were cultured with 3 × 104 MM.1S cells in the absence (■) or presence of 0.2 μM (▤), 1 μM (▧), and 5 μM (▪) VX-745 for 24 hours. IL-6 in culture supernatants was measured by ELISA.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/101/2/10.1182_blood-2002-06-1874/4/h80233671002.jpeg?Expires=1735802794&Signature=JYsJCsu9dCDB8m0vmQA2aUZxBUPIRI6oB1vAClOrK5UkK9k-3HlO3rlVm1k4nxs0SRjtgEalZ1gV2vP3-nqrkBPF2vQXt3VDbF5gB03JTCucHtQqZTmqNWmBZdv2zmqNLavLjbFg~6tBVfEnBysBoYJg88ZY~RZrKZevWEe40YhjjSZlvBRnd9Oz3AHHV7ErgYGKPsKEFlQSZDUpIm8uZVqwKtJV3mhQuAtA8FEFcZ9CvfFmrz-wiikIeTstzvg83xNEIq~l-S6lCOOXf3ziMYjz2i0dR-eZxZka60d-7UoYfMvE6NoI~j6pJx9x11LFAFpmMc3yYtazwnIJIGp3ug__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effect of VX-745 on paracrine MM cell growth in the BM.
(A) MM.1S (●), RPMI8226 (▪), and U266 (▴) (3 × 104) cells were cultured in the presence of VX-745 (0.06 μM-20 μM) for 48 hours, and cell viability was assessed by MTT assay. (B) MM.1S cells were cultured in BMSC-coated 96-well plates for 48 hours, in the absence (■) or presence of 0.2 μM (▤), 1 μM (▧), and 5 μM (▪) VX-745. DNA synthesis was assessed by [3H]TdR uptake. (C) BMSCs were cultured with 3 × 104 MM.1S cells in the absence (■) or presence of 0.2 μM (▤), 1 μM (▧), and 5 μM (▪) VX-745 for 24 hours. IL-6 in culture supernatants was measured by ELISA.
