Host CD8⁺CD4⁻NK1.1⁻ T-cell populations effect barrier function.

(A-C) B6 mice were primed to BALB.B MiHA twice, at 6 weeks and 3 weeks prior to BM transplantation. Spleen and LN cells containing 2 × 10⁷ CD3⁺ cells were isolated from these mice. Unfractionated or fractionated cells were then transferred into naive B6 animals, following depletion of CD8⁺ or NK1.1⁺ cells as described in “Materials and methods.” Resistance in these adoptively transferred mice was examined by transplanting 2 × 10⁶ BALB.B BM-TCD. Unfractionated cells from naive B6 mice were transferred as a control. Naive unfractionated versus primed CD8-depleted: P = .5536 for CFU-IL3, P = .935 for CFU-GM, andP = .576 for CFU-HPP. (D) B6 mice were primed to C3H.SW MiHA twice, at 6 weeks and 4 weeks prior to BM transplantation. Spleen and LN cells (total of 6 × 10⁷) unfractionated or fractionated by depleting CD4⁺ or CD4⁺ and CD8⁺ cells were adoptively transferred into naive B6 recipients as described in “Materials and methods.” Resistance in these mice were examined after transfer of 2 × 10⁶BM-TCD. Naive unfractionated: versus primed CD4 depleted,P = .0006, versus primed complement treated,P = .0004. Transferred populations are (1) unfractionated from naive B6, (2) unfractionated from primed B6, (3) CD8 depleted from primed B6, (4) NK1.1⁺ depleted from primed B6, (5) complement treated from primed B6, and (6) CD4 depleted from primed B6. Values represent average number of CFU ± SD.