Primed host resists MHC-matched MiHA-mismatched bone marrow allograft in the absence of TNFR1- or R2-dependent cytotoxic pathways.

B6 host resistance in the absence of TNF-α pathway was examined by transplanting BMCs from either TNFR1 or TNFR2 knock-out H-2^b MiHA-disparate donors. (A) Recipients were primed as described in “Materials and methods” with wild-type C3H.SW cells. Three weeks after priming, mice received 9.0 Gy TBI and the following day transplantation of wild-type or TNFR1-deficient marrow (2 × 10⁶ BMT-TCD). (B) Recipients were primed with spleen cells from splenectomized TNFR1-deficient H-2^b donor mice and 3 weeks after priming received 2 × 10⁶ BMT-TCD from either wild-type or the same splenectomized TNFR1-deficient donors. (C) Recipients were primed with spleen cells from splenectomized TNFR2-deficient H-2^b donor mouse and received 2 × 10⁶ BMT-TCD from either wild-type or the same splenectomized TNFR2-deficient donors. Host resistance was examined 5 days after BM-TCD transplantation by CFU-IL3 assay.