Comparison of nucleotide sequence of intron 6 of KitL gene between B6 and WB strains and usefulness of the deletion observed in the WB strain as a marker.

(A) The sequence of 450 nucleotides of 3′-splice site (−450 to −1) of intron 6. The altered nucleotides and the deleted part in the WB strain are boxed. The deleted nucleotides in WB strain are shown as dashes. The sequence of the primers used in typing of the KitL locus is indicated by arrows. These sequence data are available from GenBank under accession nos. AF516326 and AF516327. (B) Typing of the KitL locus. Using primers encompassing the deletion as a marker (indicated in panel A), the KitL locus was typed as of either B6/B6 or B6/WB. The band obtained from WB-mi/mi mice is also shown (WB/WB).