Fig. 3.
Effect of cytokine treatment on transferrin-mediated iron uptake.
Cells were treated with cytokines as described in the legend to Figure1. Uptake of transferrin bound iron (TBI) was then determined as described in “Materials and methods.” Data are shown as means ± SD for 5 independent experiments performed in duplicates and are expressed as percentage of relative iron uptake as compared to the control ( = 100%). Mean iron uptake in the control was 4.6 ± 1.8 pmol/106 cells per hour. *P < .05 compared to the control; #P < .05 when comparing cells treated with IFN-γ+LPS to cells preincubated with IL-10.
