Regulation of DMT-1 cDNA expression by cytokines and LPS.

(A) THP-1 human monocytic cells were treated with increasing concentrations of IFN-γ (1-500 U/mL), LPS (0.1-10 μg/mL), TNF-α (1-100 U/mL) or IFN-γ (10 U/mL) plus LPS (1 μg/mL), or were left untreated for 20 hours. (B) Effect of IL-10 on cytokine-induced DMT-1 mRNA expression. Cells were treated with cytokines exactly as described in the legend to Figure 1. For all experiments cytoplasmic DMT-1 mRNA levels were determined by quantitative light cycler PCR. Values were corrected for the amount of β-actin mRNA, which was determined in parallel. Results are shown as relative differences of the DMT-1/β-actin cDNA ratio as compared to the unstimulated control ( = 100%). Data are expressed as means ± SD for 3 independent experiments, each performed in triplicate. *P < .05 and **P < .01 compared with the control, respectively.