Modulation of ferroportin cDNA expression by cytokines and LPS.

(A) THP-1 human monocytic cells were treated as described in the legend to Figure 4. No significant differences from the control were found for cells treated with low amounts of TNF-α (1-10 U/mL). For all other treatments P < .01 compared with the control. (B) Effect of IL-10 on cytokine-regulated ferroportin mRNA expression. Cells were treated with cytokines exactly as described in the legend to Figure 1. In all experiments, ferroportin mRNA expression was determined by quantitative light cycler PCR. Values were then corrected for the amount of β-actin cDNA, which was determined in parallel. Results are shown as relative differences of the ferroportin/β-actin ratio as compared to the unstimulated control ( = 100%). Data are expressed as means ± SD for 3 independent experiments in each setting performed in triplicate. *P < .05 and **P < .01 compared with the control, respectively.