Transduction of naive CB CD4⁺ T cells with IL-7–displaying lentiviral vectors maintain their naive phenotype.

CD4⁺ naive CB T cells were transduced with IL-7–displaying lentiviral vectors (G/IL7SUx) or with VSV-G–pseudotyped lentiviral vectors in the presence of rIL-7 (G+rIL-7). Cells were either transduced for 3 days followed by a second transduction for 4 days (double transduction) or preactivated with rIL-7 (15 ng/mL) for 3 days and then transduced for 4 days (preactivation + single transduction). CD4⁺ adult naive T cells were preactivated with rIL-7 for 6 days and transduced for 4 days. Expression of CD45RA and EGFP was simultaneously monitored by FACS analysis 4 days after transduction. The percentages of CD45RA⁺/ EGFP⁻ and CD45RA⁺/EGFP⁺ cells are indicated.