Comparison of cell-cycle entry of naive CB T cells and naive and memory adult T cells following exposure to IL-7–displaying lentiviral vectors.

CD4⁺ naive CB (CD45RA) and naive and memory (CD45RO) adult T cells were incubated for 6 days with VSV-G–pseudotyped lentiviral vectors (G), IL7SU-displaying VSV-G–pseudotyped lentiviral vectors (G/IL7SU) or VSV-G–pseudotyped lentiviral vectors in the presence of rIL-7 (G+rIL-7). Cell-cycle progression was monitored by simultaneously visualizing the RNA (PY) and DNA (7AAD) content of these T-cell subsets. The percentages of cells in the G0/G1a, G1b, S/G2/M phase of the cell cycle are indicated in the dot blots.