Examples of FISH and IHC results.

Analyzed cases: NLPHL cases no. 4 (A), no. 13 (B,E), no. 2 (D), no. 3 (F and inset), and no. 1 (G and inset) and classical HL case no. 24 (C). Applied probes: LSI BCL6 (A-E, F inset, G inset). (D) Arrow and arrowhead indicate the der(3) t(3;7;3;1) and the der(7)t(3;7) chromosomes, respectively. (E) An example of FICTION with CD20 (blue) and LSI BCL6 (F-G and insets of both panels). Examples of sequential IHC and FISH with CD20 and LSIBCL6, and anti-BCL6 and LSI BCL6, respectively. Note the split of LSI BCL6 signals in atypical cells from NLPHL cases (A-E,F inset, and G inset) and presence of only fused signals in the cHL case (C). Original magnifications × 630 (A-C,E), × 400 (F-G), and × 1000 (F-G insets).