Combined effect of 2-ME and ATO on CLL cells in vitro.
Primary leukemia cells freshly isolated from 25 patients with CLL (A) were treated with 10 or 30 μM of 2-ME, alone or in combination with 0.5 or 1.0 μg/mL ATO for 72 hours. Cell viability was determined by MTT assay. * indicates a statistically significant difference when compared with cells exposed to 2-ME alone (P < .05). Solid bars indicate average values. (B) Fresh primary leukemia cells isolated from 17 patients with CLL were treated with 10 or 30 μM 2-ME alone or in combination with 0.5 or 1.0 μg/mL ATO for 24 hours. The O2− levels were determined by flow cytometry analysis. ∧ and # indicate a statistically significant difference when compared with the control cells (P < .05); *, a statistically significant difference when compared with cells exposed to 2-ME alone (P < .05). Error bars indicate SD of the means.
