Fig. 3.
Delayed apoptosis of PMNs inCD18−/− mice is also apparent in whole blood samples.
Flow cytometric analysis of CD16 expression on the cell surface of PMNs using the PE-labeled anti-CD16 mAb measured in whole blood samples obtained from CD18+/+(n = 13) or CD18−/− mice (n = 11). (A) Representative recording of the fluorescence histograms obtained from PMNs aged for 8 hours in culture without further stimulation. Numbers indicate apoptotic PMNs with low CD16 expression in percent of total cell number. (B) Mean values obtained after 0, 4, 8, or 22 hours of culture. Data represent means ± SDs; **P < .005.