Soluble factors in the plasma had no effect on PMN apoptosis.

Flow cytometric analysis of DNA content measured in PI-stained PMNs isolated from the circulation ofCD18^+/+ mice (left),CD18^−/− mice (middle), or the bone marrow of CD18^+/+mice (right) in the presence of RPMI medium (without FCS) supplemented with 50% plasma from CD18^+/+mice or CD18^−/−mice. Data represent apoptotic cells in percent of apoptosis in the presence of wild-type plasma (100%) 8 hours after the onset of culture. Data represent means ± SDs; NS indicates not significant.