Fig. 6.
Fig. 6. Expression of globin mRNA in transgenic mice containing the BGT133 (mGata-1/mOct-1 ATR) construct. / S1 nuclease protection analysis on day 15.5 fetal liver RNA reveals expression of the Huγ-globin transgene in 7/7 single-copy and 10/10 multicopy animals, to a mean of 37% of each endogenous mouse βmajor-globin gene. NT, nontransgenic; Huγ, human γ-globin protected probe fragment; Moβ, mouse βmajor-globin protected probe fragment; BGT50 T225, a heterozygous BGT50 transgenic line; 3X, probe excess control using 3 μg of fetal liver RNA; copy number = 1*, one intact and one partial copy of the transgene.

Expression of globin mRNA in transgenic mice containing the BGT133 (mGata-1/mOct-1 ATR) construct.

S1 nuclease protection analysis on day 15.5 fetal liver RNA reveals expression of the Huγ-globin transgene in 7/7 single-copy and 10/10 multicopy animals, to a mean of 37% of each endogenous mouse βmajor-globin gene. NT, nontransgenic; Huγ, human γ-globin protected probe fragment; Moβ, mouse βmajor-globin protected probe fragment; BGT50 T225, a heterozygous BGT50 transgenic line; 3X, probe excess control using 3 μg of fetal liver RNA; copy number = 1*, one intact and one partial copy of the transgene.

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