Bcr-Abl inhibition of G-CSF–induced granulocytic differentiation in 32Dcl3 cells by a kinase-dependent mechanism.

(A) Abl (top panel) and phosphotyrosine (bottom panel) immunoblots of lysates from either 32Dcl3, 32D_Bcr-Ablwt or 32D_{Bcr-AblK1172R} cells. (B) Morphological differentiation of 32Dcl3, 32D_Bcr-Ablwt, or 32D_{Bcr-AblK1172R} cells 8 days after stimulation with G-CSF as assessed by May-Gruenwald-Giemsa staining (original magnification, × 400). Cells grown in the presence of IL-3 are shown as a control. (C) Expression of Gr-1 on 32Dcl3, 32D_Bcr-Ablwt, or 32D_{Bcr-AblK1172R} cells upon G-CSF stimulation. Gr-1 expression on cells grown in the presence of IL-3 is shown as a control.